PrimePCR™ SYBR® Green Assay: TNIP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027718

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010752
Assay Design:   Intron-spanning
Chromosome Location:   4:2747179-2749444question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000510267 ENST00000315423 ENST00000503235

Gene Information

TNIP2 binds to the C-terminal zinc finger domain of A20 (TNFAIP3; MIM 191163) and is involved in activation of the ERK (see MAPK3; MIM 601795) MAP kinase pathway in various cell types (Van Huffel et al. 2001 [PubMed 11390377]; Papoutsopoulou et al. 2006 [PubMed 16633345]).[supplied by OMIM Mar 2008]

Gene Symbol:   TNIP2
Gene Name:   TNFAIP3 interacting protein 2
Aliases:   ABIN2, DKFZp434J1313, FLIP1, KLIP, MGC4289
RefSeq:   NC_000004.11 NT_006051.18
Ensembl:   ENSG00000168884
Entrez:   79155
UniGene:   Hs.726088
Chromosome Mapping:   4p16.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 33.620000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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