PrimePCR™ SYBR® Green Assay: C11orf54, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010737
Assay Design:   Intron-spanning
Chromosome Location:   11:93483539-93486905question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000528288 ENST00000331239 ENST00000533585 ENST00000528099 ENST00000354421 ENST00000530620 ENST00000527003 ENST00000531650 ENST00000530279 ENST00000524485 ENST00000527363 ENST00000526335 ENST00000540113

Gene Information

Description Not Available

Gene Symbol:   C11orf54
Gene Name:   chromosome 11 open reading frame 54
Aliases:   PTD012
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000182919
Entrez:   28970
UniGene:   Hs.8360
Chromosome Mapping:   11q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.310000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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