PrimePCR™ SYBR® Green Assay: FAM54B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: FAM54B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM54B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010734
Assay Design:   Intron-spanning
Chromosome Location:   1:26150157-26152835question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000424294 ENST00000533762 ENST00000529116 ENST00000474295 ENST00000488327 ENST00000472643 ENST00000526894 ENST00000524618 ENST00000374307 ENST00000525713 ENST00000374301 ENST00000526158 ENST00000374300 ENST00000466284 ENST00000374303

Gene Information

Description Not Available

Gene Symbol:   FAM54B
Gene Name:   family with sequence similarity 54, member B
Aliases:   MST116, MSTP116
RefSeq:   NC_000001.10 NG_009930.1 NT_004610.19
Ensembl:   ENSG00000117640
Entrez:   56181
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download