PrimePCR™ SYBR® Green Assay: WNK1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027708

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010732
Assay Design:   Intron-spanning
Chromosome Location:   12:991134-992178question
Amplicon Length:   146
Splice Variants Targeted:   ENST00000535572 ENST00000315939 ENST00000544965 ENST00000537687 ENST00000530271 ENST00000340908 ENST00000252477 ENST00000537501

Gene Information

This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq May 2010]

Gene Symbol:   WNK1
Gene Name:   WNK lysine deficient protein kinase 1
Aliases:   HSAN2, HSN2, KDP, KIAA0344, MGC163339, MGC163341, PRKWNK1, PSK, p65
RefSeq:   NC_000012.11 NG_017078.1 NT_009759.16 NG_007984.2
Ensembl:   ENSG00000060237
Entrez:   65125
UniGene:   Hs.731621
Chromosome Mapping:   12p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.220000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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