PrimePCR™ SYBR® Green Assay: HAND2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027698

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010704
Assay Design:   Intron-spanning
Chromosome Location:   4:174448485-174449950question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000359562 ENST00000393686 ENST00000535864

Gene Information

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members the HAND proteins which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion they function in the formation of the right ventricle and aortic arch arteries implicating them as mediators of congenital heart disease. In addition this transcription factor plays an important role in limb and branchial arch development. [provided by RefSeq Jul 2008]

Gene Symbol:   HAND2
Gene Name:   heart and neural crest derivatives expressed 2
Aliases:   DHAND2, FLJ16260, Hed, MGC125303, MGC125304, Thing2, bHLHa26, dHand
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164107
Entrez:   9464
UniGene:   Hs.388245
Chromosome Mapping:   4q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 32.960000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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