This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027685
PrimePCR™ PreAmp for SYBR® Green Assay: GPR161, Human
PrimePCR™ Template for SYBR® Green Assay: GPR161, Human
Upon ligand binding G protein-coupled receptors such as GPR161 activate cytoplasmic G proteins (see GNAS MIM 139320) allowing the receptors to transduce extracellular signals across the plasma membrane into the cell. Phosphorylation of the receptor attenuates signaling (Matteson et al. 2008 [PubMed 18250320]).[supplied by OMIM Aug 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.