PrimePCR™ SYBR® Green Assay: C7orf44, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010666
Assay Design:   Intron-spanning
Chromosome Location:   7:43684932-43687192question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000395879 ENST00000310564 ENST00000415798 ENST00000431651 ENST00000395880 ENST00000223336

Gene Information

Description Not Available

Gene Symbol:   C7orf44
Gene Name:   chromosome 7 open reading frame 44
Aliases:   FLJ10803
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000106603
Entrez:   55744
UniGene:   Hs.655165
Chromosome Mapping:   7p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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