PrimePCR™ SYBR® Green Assay: POLE3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027673

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010643
Assay Design:   Intron-spanning
Chromosome Location:   9:116171160-116171954question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000374171 ENST00000374169

Gene Information

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription replication and packaging.[supplied by OMIM Apr 2004]

Gene Symbol:   POLE3
Gene Name:   polymerase (DNA directed), epsilon 3 (p17 subunit)
Aliases:   CHARAC17, CHRAC17, YBL1, p17
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000148229
Entrez:   54107
UniGene:   Hs.108112
Chromosome Mapping:   9q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.230000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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