PrimePCR™ SYBR® Green Assay: TMEM18, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027668

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010633
Assay Design:   Intron-spanning
Chromosome Location:   2:669757-675531question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000281017 ENST00000405941 ENST00000355654

Gene Information

Description Not Available

Gene Symbol:   TMEM18
Gene Name:   transmembrane protein 18
Aliases:   DKFZp434C1714
RefSeq:   NC_000002.11 NT_022221.13
Ensembl:   ENSG00000151353
Entrez:   129787
UniGene:   Hs.43899
Chromosome Mapping:   2p25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.560000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download