PrimePCR™ SYBR® Green Assay: LYRM4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027667

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010631
Assay Design:   Intron-spanning
Chromosome Location:   6:5216933-5260969question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000330636 ENST00000464010 ENST00000480566 ENST00000500576

Gene Information

Description Not Available

Gene Symbol:   LYRM4
Gene Name:   LYR motif containing 4
Aliases:   C6orf149, CGI-203, ISD11
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000214113
Entrez:   57128
UniGene:   Hs.387755
Chromosome Mapping:   6p25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 33.920000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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