PrimePCR™ SYBR® Green Assay: EPS8L2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010627
Assay Design:   Intron-spanning
Chromosome Location:   11:709573-710485question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000524763 ENST00000318562 ENST00000533256 ENST00000534755 ENST00000533500 ENST00000531348 ENST00000530636 ENST00000526198

Gene Information

This gene encodes a member of the EPS8 gene family. The encoded protein like other members of the family is thought to link growth factor stimulation to actin organization generating functional redundancy in the pathways that regulate actin cytoskeletal remodeling. [provided by RefSeq Dec 2008]

Gene Symbol:   EPS8L2
Gene Name:   EPS8-like 2
Aliases:   EPS8R2, FLJ16738, FLJ21935, FLJ22171, MGC126530, MGC3088
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000177106
Entrez:   64787
UniGene:   Hs.55016
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.270000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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