PrimePCR™ SYBR® Green Assay: TSEN34, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027664

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TSEN34, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TSEN34, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010625
Assay Design:   Intron-spanning
Chromosome Location:   19:54696128-54697099question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000302937 ENST00000396383 ENST00000396388 ENST00000429671

Gene Information

This gene encodes a catalytic subunit of the tRNA splicing endonuclease which catalyzes the removal of introns from precursor tRNAs. The endonuclease complex is also associated with a pre-mRNA 3-prime end processing factor. A mutation in this gene results in the neurological disorder pontocerebellar hypoplasia type 2. Multiple alternatively spliced variants encoding the same protein have been identified.[provided by RefSeq Oct 2009]

Gene Symbol:   TSEN34
Gene Name:   tRNA splicing endonuclease 34 homolog (S. cerevisiae)
Aliases:   LENG5, PCH2C, SEN34, SEN34L
RefSeq:   NC_000019.9 NT_011109.16 NG_015810.1
Ensembl:   ENSG00000170892
Entrez:   79042
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.970000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download