PrimePCR™ SYBR® Green Assay: IFT122, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027659

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010617
Assay Design:   Intron-spanning
Chromosome Location:   3:129168722-129170796question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000347300 ENST00000296266 ENST00000507564 ENST00000504021 ENST00000349441 ENST00000348417 ENST00000514275 ENST00000508826 ENST00000512157 ENST00000515783 ENST00000446384 ENST00000454840 ENST00000431818 ENST00000440957

Gene Information

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression signal transduction apoptosis and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   IFT122
Gene Name:   intraflagellar transport 122 homolog (Chlamydomonas)
Aliases:   CED, SPG, WDR10, WDR10p, WDR140
RefSeq:   NC_000003.11 NT_005612.16 NG_023392.1
Ensembl:   ENSG00000163913
Entrez:   55764
UniGene:   Hs.655284
Chromosome Mapping:   3q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 33.650000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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