This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: MBD4, Human
PrimePCR™ Template for SYBR® Green Assay: MBD4, Human
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2 MBD1 MBD2 MBD3 and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins with the exception of MBD3 is capable of binding specifically to methylated DNA. MBD4 may function to mediate the biological consequences of the methylation signal. In addition MBD4 has protein sequence similarity to bacterial DNA repair enzymes and thus may have some function in DNA repair. Further MBD4 gene mutations are detected in tumors with primary microsatellite-instability (MSI) a form of genomic instability associated with defective DNA mismatch repair and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.