PrimePCR™ SYBR® Green Assay: CYP1A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010608
Assay Design:   Intron-spanning
Chromosome Location:   15:75013932-75014666question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000379727 ENST00000395048 ENST00000395049 ENST00000268062

Gene Information

This gene CYP1A1 encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs) some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however it is able to metabolize some PAHs to carcinogenic intermediates. The gene has been associated with lung cancer risk. A related family member CYP1A2 is located approximately 25 kb away from CYP1A1 on chromosome 15. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP1A1
Gene Name:   cytochrome P450, family 1, subfamily A, polypeptide 1
Aliases:   AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX
RefSeq:   NC_000015.9 NG_008431.1 NT_010194.17
Ensembl:   ENSG00000140465
Entrez:   1543
UniGene:   Hs.72912
Chromosome Mapping:   15q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.720000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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