PrimePCR™ SYBR® Green Assay: WDFY3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027650

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010593
Assay Design:   Intron-spanning
Chromosome Location:   4:85614084-85617160question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000295888 ENST00000514711 ENST00000322366

Gene Information

This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However it is not necessary for starvation-induced macroautophagy. [provided by RefSeq May 2010]

Gene Symbol:   WDFY3
Gene Name:   WD repeat and FYVE domain containing 3
Aliases:   ALFY, KIAA0993, MGC16461, ZFYVE25
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000163625
Entrez:   23001
UniGene:   Hs.480116
Chromosome Mapping:   4q21.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.270000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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