PrimePCR™ SYBR® Green Assay: C2orf63, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010591
Assay Design:   Intron-spanning
Chromosome Location:   2:55404863-55407682question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000407122 ENST00000401408 ENST00000406076 ENST00000406437

Gene Information

Description Not Available

Gene Symbol:   C2orf63
Gene Name:   chromosome 2 open reading frame 63
Aliases:   FLJ31438
RefSeq:   NG_017017.1 NC_000002.11 NT_022184.15
Ensembl:   ENSG00000162994
Entrez:   130162
UniGene:   Hs.347014
Chromosome Mapping:   2p16.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.140000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download