PrimePCR™ SYBR® Green Assay: C15orf40, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027648

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010586
Assay Design:   Intron-spanning
Chromosome Location:   15:83677380-83679093question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000513601 ENST00000538348 ENST00000451195 ENST00000304177

Gene Information

Description Not Available

Gene Symbol:   C15orf40
Gene Name:   chromosome 15 open reading frame 40
Aliases:   FLJ33606, MGC29937
RefSeq:   NC_000015.9 NT_077661.3
Ensembl:   ENSG00000169609
Entrez:   123207
UniGene:   Hs.352541
Chromosome Mapping:   15q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.530000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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