This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027638
PrimePCR™ PreAmp for SYBR® Green Assay: CANX, Human
PrimePCR™ Template for SYBR® Green Assay: CANX, Human
This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.