PrimePCR™ SYBR® Green Assay: NKTR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010534
Assay Design:   Intron-spanning
Chromosome Location:   3:42642511-42659112question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000232978 ENST00000442970 ENST00000445842

Gene Information

This gene encodes a membrane-anchored protein with a hydrophobic amino terminal domain and a cyclophilin-like PPIase domain. It is present on the surface of natural killer cells and facilitates their binding to targets. Its expression is regulated by IL2 activation of the cells. [provided by RefSeq Jul 2008]

Gene Symbol:   NKTR
Gene Name:   natural killer-tumor recognition sequence
Aliases:   DKFZp686F1754, DKFZp686G0426, DKFZp686J06106, DKFZp686N24126, MGC90527, p104
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000114857
Entrez:   4820
UniGene:   Hs.529509
Chromosome Mapping:   3p23-p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 33.700000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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