PrimePCR™ SYBR® Green Assay: SIL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SIL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SIL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010476
Assay Design:   Intron-spanning
Chromosome Location:   5:138456814-138463471question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000394817 ENST00000265195 ENST00000509534 ENST00000508639 ENST00000513453 ENST00000507002 ENST00000505830 ENST00000505353 ENST00000537511

Gene Information

This gene encodes a resident endoplasmic reticulum (ER) N-linked glycoprotein with an N-terminal ER targeting sequence 2 putative N-glycosylation sites and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   SIL1
Gene Name:   SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
Aliases:   BAP, MSS, ULG5
RefSeq:   NC_000005.9 NG_008112.1 NT_034772.6
Ensembl:   ENSG00000120725
Entrez:   64374
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 33.930000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download