PrimePCR™ SYBR® Green Assay: TKT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027589

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010447
Assay Design:   Intron-spanning
Chromosome Location:   3:53260848-53262149question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000462138 ENST00000423525 ENST00000423516 ENST00000296289 ENST00000414014

Gene Information

This gene encodes a thiamine-dependent enzyme which plays a role in the channeling of excess sugar phosphates to glycolysis in the pentose phosphate pathway. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq Sep 2010]

Gene Symbol:   TKT
Gene Name:   transketolase
Aliases:   FLJ34765, TK, TKT1
RefSeq:   NC_000003.11 NT_022517.18 NG_027815.1
Ensembl:   ENSG00000163931
Entrez:   7086
UniGene:   Hs.89643
Chromosome Mapping:   3p14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.030000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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