PrimePCR™ SYBR® Green Assay: CRYZL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010399
Assay Design:   Intron-spanning
Chromosome Location:   21:34974589-34975739question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000381554 ENST00000290244 ENST00000381540 ENST00000445393 ENST00000361534 ENST00000440526 ENST00000414079 ENST00000426935 ENST00000431177 ENST00000452332

Gene Information

This gene encodes a protein that has sequence similarity to zeta crystallin also known as quinone oxidoreductase. This zeta crystallin-like protein also contains an NAD(P)H binding site. Alternatively spliced transcript variants have been observed but their full-length nature has not been completely determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CRYZL1
Gene Name:   crystallin, zeta (quinone reductase)-like 1
Aliases:   4P11, DKFZp434M035, QOH-1
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000205758
Entrez:   9946
UniGene:   Hs.352671
Chromosome Mapping:   21q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.750000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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