PrimePCR™ SYBR® Green Assay: PCID2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027565

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PCID2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PCID2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010393
Assay Design:   Intron-spanning
Chromosome Location:   13:113835488-113838750question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000337344 ENST00000375479 ENST00000375477 ENST00000246505 ENST00000375459 ENST00000375457 ENST00000351317 ENST00000375462 ENST00000246506

Gene Information

PCID2 is expressed in immature and early-stage B lymphocytes and regulates expression of the mitotic checkpoint protein MAD2 (MAD2L1; MIM 601467) (Nakaya et al. 2010 [PubMed 20870947]).[supplied by OMIM Jan 2011]

Gene Symbol:   PCID2
Gene Name:   PCI domain containing 2
Aliases:   DKFZp686C20226, F10, FLJ11305, FLJ99362, MGC16774
RefSeq:   NC_000013.10 NT_027140.6
Ensembl:   ENSG00000126226
Entrez:   55795
Chromosome Mapping:   13q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 33.210000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download