This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027552
PrimePCR™ PreAmp for SYBR® Green Assay: CLK2, Human
PrimePCR™ Template for SYBR® Green Assay: CLK2, Human
This gene encodes a member of the CLK family of dual specificity protein kinases. CLK family members have been shown to interact with and phosphorylate serine- and arginine-rich (SR) proteins of the spliceosomal complex which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. Note that this gene is distinct from TELO2 gene (GeneID:9894) which shares CLK2 and hCLK2 symbol aliases in common with this gene but encodes a protein that is involved in telomere length regulation. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.