This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: ERRFI1, Human
PrimePCR™ Template for SYBR® Green Assay: ERRFI1, Human
ERRFI1 is a cytoplasmic protein whose expression is upregulated with cell growth (Wick et al. 1995 [PubMed 7641805]). It shares significant homology with the protein product of rat gene-33 which is induced during cell stress and mediates cell signaling (Makkinje et al. 2000 [PubMed 10749885]; Fiorentino et al. 2000 [PubMed 11003669]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.