This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027508
PrimePCR™ PreAmp for SYBR® Green Assay: PPP6R2, Human
PrimePCR™ Template for SYBR® Green Assay: PPP6R2, Human
Protein phosphatase regulatory subunits such as SAPS2 modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity recruiting substrates and determining the intracellular localization of the holoenzyme. SAPS2 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan 2006 [PubMed 16769727]).[supplied by OMIM Nov 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.