PrimePCR™ SYBR® Green Assay: GHRHR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027499

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GHRHR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GHRHR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010236
Assay Design:   Intron-spanning
Chromosome Location:   7:31016896-31018844question
Amplicon Length:   123
Splice Variants Targeted:   ENST00000326139 ENST00000409904 ENST00000409233 ENST00000409316 ENST00000337750

Gene Information

This gene encodes a receptor for growth hormone-releasing hormone. Binding of this hormone to the receptor leads to synthesis and release of growth hormone. Mutations in this gene have been associated with isolated growth hormone deficiency (IGHD) also known as Dwarfism of Sindh a disorder characterized by short stature. [provided by RefSeq Jun 2010]

Gene Symbol:   GHRHR
Gene Name:   growth hormone releasing hormone receptor
Aliases:   GHRFR, GRFR, IGHD1B
RefSeq:   NC_000007.13 NG_021416.1 NT_007819.17
Ensembl:   ENSG00000106128
Entrez:   2692
Chromosome Mapping:   7p14

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.190000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download