This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: NOSTRIN, Human
PrimePCR™ Template for SYBR® Green Assay: NOSTRIN, Human
Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission inflammatory response and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production endothelial NO synthase (ENOS; MIM 163729) and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures thereby attenuating ENOS-dependent NO production.[supplied by OMIM Apr 2004]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.