PrimePCR™ SYBR® Green Assay: PPIL2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010214
Assay Design:   Intron-spanning
Chromosome Location:   22:22043115-22048897question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000335025 ENST00000398831 ENST00000406385 ENST00000446951 ENST00000412327 ENST00000456792 ENST00000492445

Gene Information

This gene is a member of the cyclophilin family of peptidylprolyl isomerases. The cyclophilins are a highly conserved ubiquitous family members of which play an important role in protein folding immunosuppression by cyclosporin A and infection of HIV-1 virions. This protein interacts with the proteinase inhibitor eglin c and is localized in the nucleus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PPIL2
Gene Name:   peptidylprolyl isomerase (cyclophilin)-like 2
Aliases:   CYC4, CYP60, Cyp-60, FLJ39930, MGC33174, MGC787, hCyP-60
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100023
Entrez:   23759
UniGene:   Hs.438587
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.540000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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