PrimePCR™ SYBR® Green Assay: TMC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010199
Assay Design:   Intron-spanning
Chromosome Location:   9:75407134-75420332question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000297784 ENST00000396235 ENST00000537917 ENST00000538054 ENST00000340019 ENST00000396237 ENST00000542143

Gene Information

This gene is considered a member of a gene family predicted to encode transmembrane proteins. The specific function of this gene is unknown; however it is known to be required for normal function of cochlear hair cells. Mutations in this gene have been associated with progressive postlingual hearing loss and profound prelingual deafness. [provided by RefSeq Jul 2008]

Gene Symbol:   TMC1
Gene Name:   transmembrane channel-like 1
Aliases:   DFNA36, DFNB11, DFNB7
RefSeq:   NC_000009.11 NG_008213.1 NT_008470.19
Ensembl:   ENSG00000165091
Entrez:   117531
UniGene:   Hs.670211
Chromosome Mapping:   9q21.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.270000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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