PrimePCR™ SYBR® Green Assay: PARVA, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027486

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010191
Assay Design:   Intron-spanning
Chromosome Location:   11:12518098-12525912question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000334956 ENST00000528916 ENST00000539723 ENST00000550549 ENST00000538608

Gene Information

This gene encodes a member of the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion motility and survival. [provided by RefSeq Dec 2010]

Gene Symbol:   PARVA
Gene Name:   parvin, alpha
Aliases:   CH-ILKBP, FLJ10793, FLJ12254, FLJ25106, FLJ46049, MXRA2
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000197702
Entrez:   55742
UniGene:   Hs.432914
Chromosome Mapping:   11p15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.230000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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