This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027485
PrimePCR™ PreAmp for SYBR® Green Assay: TAB2, Human
PrimePCR™ Template for SYBR® Green Assay: TAB2, Human
The protein encoded by this gene is an activator of MAP3K7/TAK1 which is required for for the IL-1 induced activation of nuclear factor kappaB and MAPK8/JNK. This protein forms a kinase complex with TRAF6 MAP3K7 and TAB1 thus serves as an adaptor linking MAP3K7 and TRAF6. This protein TAB1 and MAP3K7 also participate in the signal transduction induced by TNFSF11/RANKl through the activation of the receptor activator of NF-kappB (TNFRSF11A/RANK) which may regulate the development and function of osteoclasts. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.