PrimePCR™ SYBR® Green Assay: RNASET2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027480

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010177
Assay Design:   Intron-spanning
Chromosome Location:   6:167343222-167344599question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000366855 ENST00000508775 ENST00000476238 ENST00000478180 ENST00000428859

Gene Information

This ribonuclease gene is a novel member of the Rh/T2/S-glycoprotein class of extracellular ribonucleases. It is a single copy gene that maps to 6q27 a region associated with human malignancies and chromosomal rearrangement. [provided by RefSeq Jul 2008]

Gene Symbol:   RNASET2
Gene Name:   ribonuclease T2
Aliases:   FLJ42372, RNASE6PL, bA514O12.3
RefSeq:   NC_000006.11 NG_016280.1 NT_025741.15
Ensembl:   ENSG00000026297
Entrez:   8635
UniGene:   Hs.529989
Chromosome Mapping:   6q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 34.590000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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