PrimePCR™ SYBR® Green Assay: HOOK1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010120
Assay Design:   Intron-spanning
Chromosome Location:   1:60287568-60294493question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000455990 ENST00000371208 ENST00000395561

Gene Information

This gene encodes a member of the hook family of coiled-coil proteins which bind to microtubules and organelles through their N- and C-terminal domains respectively. The encoded protein localizes to discrete punctuate subcellular structures and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   HOOK1
Gene Name:   hook homolog 1 (Drosophila)
Aliases:   HK1, MGC10642
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000134709
Entrez:   51361
UniGene:   Hs.378836
Chromosome Mapping:   1p32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.670000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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