This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: INCENP, Human
PrimePCR™ Template for SYBR® Green Assay: INCENP, Human
In mammalian cells 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' or transiently interacting proteins (reviewed by Choo 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139) CENPB (MIM 117140) CENPC1 (MIM 117141) and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g. MIM 600112); CliPs (e.g. MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke 1991 [PubMed 1860899]) the initial members of the passenger protein group display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase the proteins are located within the midbody in the intercellular bridge where they are discarded after cytokinesis (Cutts et al. 1999 [PubMed 10369859]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.