This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: TLN2, Human
PrimePCR™ Template for SYBR® Green Assay: TLN2, Human
This gene encodes a protein related to talin 1 a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but like talin 1 is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.