PrimePCR™ SYBR® Green Assay: TLN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010095
Assay Design:   Intron-spanning
Chromosome Location:   15:63131104-63132741question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000306829

Gene Information

This gene encodes a protein related to talin 1 a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but like talin 1 is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq Jul 2008]

Gene Symbol:   TLN2
Gene Name:   talin 2
Aliases:   DKFZp451B1011, DKFZp686I0976, DKFZp686K0979, ILWEQ, KIAA0320
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000171914
Entrez:   83660
UniGene:   Hs.569438
Chromosome Mapping:   15q15-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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