PrimePCR™ SYBR® Green Assay: ISY1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010069
Assay Design:   Intron-spanning
Chromosome Location:   3:128864652-128875710question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000418265 ENST00000393295 ENST00000273541 ENST00000393292

Gene Information

Description Not Available

Gene Symbol:   ISY1
Gene Name:   ISY1 splicing factor homolog (S. cerevisiae)
Aliases:   FSAP33, KIAA1160
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000240682
Entrez:   57461
UniGene:   Hs.512661
Chromosome Mapping:   3q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.290000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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