PrimePCR™ SYBR® Green Assay: YSK4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027436

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010046
Assay Design:   Intron-spanning
Chromosome Location:   2:135757525-135763085question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000375845 ENST00000375844 ENST00000392918 ENST00000392915 ENST00000425952 ENST00000392917 ENST00000315513

Gene Information

Description Not Available

Gene Symbol:   YSK4
Gene Name:   YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae)
Aliases:   FLJ23074, RCK
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000176601
Entrez:   80122
UniGene:   Hs.659395
Chromosome Mapping:   2q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.720000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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