PrimePCR™ SYBR® Green Assay: PRRC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027433

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010043
Assay Design:   Intron-spanning
Chromosome Location:   5:126865993-126869351question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000296666 ENST00000442138 ENST00000512635 ENST00000512535 ENST00000330542 ENST00000414018

Gene Information

Description Not Available

Gene Symbol:   PRRC1
Gene Name:   proline-rich coiled-coil 1
Aliases:   FLJ32875
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000164244
Entrez:   133619
UniGene:   Hs.483259
Chromosome Mapping:   5q23.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.720000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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