PrimePCR™ SYBR® Green Assay: CDYL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027429

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010026
Assay Design:   Intron-spanning
Chromosome Location:   6:4952591-4954205question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000328908 ENST00000397588 ENST00000449732 ENST00000343762

Gene Information

Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain a motif implicated in chromatin binding and gene suppression and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CDYL
Gene Name:   chromodomain protein, Y-like
Aliases:   CDYL1, DKFZp586C1622, MGC131936
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000153046
Entrez:   9425
UniGene:   Hs.269092
Chromosome Mapping:   6p25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 34.870000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download