PrimePCR™ SYBR® Green Assay: GTF2H3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010018
Assay Design:   Intron-spanning
Chromosome Location:   12:124137079-124139521question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000539994 ENST00000538845 ENST00000228955 ENST00000543341 ENST00000543154 ENST00000542231

Gene Information

Description Not Available

Gene Symbol:   GTF2H3
Gene Name:   general transcription factor IIH, polypeptide 3, 34kDa
Aliases:   BTF2, TFB4, TFIIH
RefSeq:   NC_000012.11 NG_015862.1 NT_009755.19
Ensembl:   ENSG00000111358
Entrez:   2967
UniGene:   Hs.355348
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.360000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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