This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027410
PrimePCR™ PreAmp for SYBR® Green Assay: STARD7, Human
PrimePCR™ Template for SYBR® Green Assay: STARD7, Human
Although the function of this gene is not known its existence is supported by mRNA and EST data. The predicted gene product contains a region similar to the STAR-related lipid transfer (START) domain which is often present in proteins involved in the cell signaling mediated by lipid binding. Alternatively spliced transcript variants have been described although some transcripts occur only in cancer cell lines. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.