PrimePCR™ SYBR® Green Assay: DUSP15, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009971
Assay Design:   Intron-spanning
Chromosome Location:   20:30454887-30458137question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000278979 ENST00000398084 ENST00000339738 ENST00000486996 ENST00000375966 ENST00000398083

Gene Information

The protein encoded by this gene belongs to the non-receptor class of the protein-tyrosine phosphatase family. The encoded protein has both protein-tyrosine phophatase activity and serine/threonine-specific phosphatase activity and therefore is known as a dual specificity phosphatase. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP15
Gene Name:   dual specificity phosphatase 15
Aliases:   FLJ40111, VHY
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000149599
Entrez:   128853
UniGene:   Hs.585017
Chromosome Mapping:   20q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.500000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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