PrimePCR™ SYBR® Green Assay: FMNL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027402

List Price:    $142.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009963
Assay Design:   Intron-spanning
Chromosome Location:   17:43308003-43310599question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000328118 ENST00000331495

Gene Information

This gene encodes a formin-related protein. Formin-related proteins have been implicated in morphogenesis cytokinesis and cell polarity. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   FMNL1
Gene Name:   formin-like 1
Aliases:   C17orf1, C17orf1B, FHOD4, FMNL, KW-13, MGC133052, MGC1894, MGC21878
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000184922
Entrez:   752
UniGene:   Hs.100217
Chromosome Mapping:   17q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.610000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download