PrimePCR™ SYBR® Green Assay: CXorf59, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009953
Assay Design:   Intron-spanning
Chromosome Location:   X:36156502-36162736question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000378660 ENST00000397458 ENST00000313548

Gene Information

While this gene is well-supported by transcript data no functional information on its protein product is currently available. [provided by RefSeq Dec 2009]

Gene Symbol:   CXorf59
Gene Name:   chromosome X open reading frame 59
Aliases:   FLJ36601, MGC126747, MGC126749
RefSeq:   NC_000023.10 NG_016381.1 NT_167197.1
Ensembl:   ENSG00000176034
Entrez:   286464
UniGene:   Hs.376425
Chromosome Mapping:   Xp21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.860000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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