PrimePCR™ SYBR® Green Assay: GRIK1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0027396

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009947
Assay Design:   Intron-spanning
Chromosome Location:   21:31062237-31066313question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000327783 ENST00000389125 ENST00000399913 ENST00000399914 ENST00000389124 ENST00000399907 ENST00000399909 ENST00000535441 ENST00000309434

Gene Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain which is thought to alter the properties of ion flow. Alternative splicing resulting in transcript variants encoding different isoforms has been noted for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   GRIK1
Gene Name:   glutamate receptor, ionotropic, kainate 1
Aliases:   EAA3, EEA3, GLR5, GLUR5
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000171189
Entrez:   2897
UniGene:   Hs.664641
Chromosome Mapping:   21q22.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.820000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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