PrimePCR™ SYBR® Green Assay: ALDH6A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009943
Assay Design:   Intron-spanning
Chromosome Location:   14:74531996-74533449question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000555126 ENST00000350259 ENST00000553458

Gene Information

This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene a mitochondrial methylmalonate semialdehyde dehydrogenase catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine 3-hydroxypropionic acid and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. [provided by RefSeq Jul 2008]

Gene Symbol:   ALDH6A1
Gene Name:   aldehyde dehydrogenase 6 family, member A1
Aliases:   MGC40271, MMSADHA, MMSDH
RefSeq:   NC_000014.8 NG_012257.1 NT_026437.12
Ensembl:   ENSG00000119711
Entrez:   4329
UniGene:   Hs.293970
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.330000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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