PrimePCR™ SYBR® Green Assay: BRF2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0009906
Assay Design:   Intron-spanning
Chromosome Location:   8:37702703-37704422question
Amplicon Length:   50
Splice Variants Targeted:   ENST00000220659 ENST00000520601 ENST00000545765

Gene Information

This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene a TFIIB-like factor is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq Jul 2008]

Gene Symbol:   BRF2
Gene Name:   BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
Aliases:   BRFU, FLJ11052, TFIIIB50
RefSeq:   NC_000008.10 NT_167187.1
Ensembl:   ENSG00000104221
Entrez:   55290
UniGene:   Hs.709301
Chromosome Mapping:   8p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.890000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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