This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0027364
PrimePCR™ PreAmp for SYBR® Green Assay: CPAMD8, Human
PrimePCR™ Template for SYBR® Green Assay: CPAMD8, Human
CPAMD8 belongs to the complement component-3 (C3; MIM 120700)/alpha-2-macroglobulin (A2M; MIM 103950) family of proteins which are involved in innate immunity and damage control. Complement components recognize and eliminate pathogens by direct binding or by mediating opsonization/phagocytosis and intracellular killing and A2M is a broad-spectrum protease inhibitor (Li et al. 2004 [PubMed 15177561]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.